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National Research Council Canada human non-small-cell lung cancer cell line a549
Effect of D. japonica Mucus Treatment on Cell Growth. ( a ) A representative experiment demonstrating the dose-dependent impact of mucus treatment on NCI-H358 and 16HBE14o cell numbers, assessed via crystal violet assay 48 h post-treatment. Each point reflects the mean value (±SD) of three independent samples, normalized to their respective controls. ** = p < 0.01, calculated using Student’s t -test. The inset displays EC50 values determined using the Graphpad Prism program. ( b ) This representative experiment illustrates the influence of various mucus preparations on NCI-H358 cell growth, evaluated through crystal violet assay 48 h post-treatment. Each bar represents the mean absorbance (±SD) of three independent samples. NS indicates not significant, determined by one-way ANOVA. ( c ) A representative experiment illustrating the dose-dependent impact of mucus treatment on cell numbers in <t>A549,</t> Caco-2, and THP-1 cells. Cell numbers were assessed using the crystal violet assay for A549 and Caco-2 cells, and direct counting for THP-1 cells, 48 h post-treatment. Each bar represents the mean value (±SD) of three independent samples, normalized to their respective controls. ** = p < 0.01, calculated by Student’s t -test. ( d ) Representative experiment, in which we observe the dose-dependent effect of mucus treatment on cell death, evaluated using the PI incorporation assay in A549, Caco-2, and THP-1 cells, 48 h post-treatment. Each bar represents the percentage of dead and alive cells (±SD) assessed in three independent samples. * = p < 0.05; ** = p < 0.01, determined by Student’s t -test.
Human Non Small Cell Lung Cancer Cell Line A549, supplied by National Research Council Canada, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effect of D. japonica Mucus Treatment on Cell Growth. ( a ) A representative experiment demonstrating the dose-dependent impact of mucus treatment on NCI-H358 and 16HBE14o cell numbers, assessed via crystal violet assay 48 h post-treatment. Each point reflects the mean value (±SD) of three independent samples, normalized to their respective controls. ** = p < 0.01, calculated using Student’s t -test. The inset displays EC50 values determined using the Graphpad Prism program. ( b ) This representative experiment illustrates the influence of various mucus preparations on NCI-H358 cell growth, evaluated through crystal violet assay 48 h post-treatment. Each bar represents the mean absorbance (±SD) of three independent samples. NS indicates not significant, determined by one-way ANOVA. ( c ) A representative experiment illustrating the dose-dependent impact of mucus treatment on cell numbers in A549, Caco-2, and THP-1 cells. Cell numbers were assessed using the crystal violet assay for A549 and Caco-2 cells, and direct counting for THP-1 cells, 48 h post-treatment. Each bar represents the mean value (±SD) of three independent samples, normalized to their respective controls. ** = p < 0.01, calculated by Student’s t -test. ( d ) Representative experiment, in which we observe the dose-dependent effect of mucus treatment on cell death, evaluated using the PI incorporation assay in A549, Caco-2, and THP-1 cells, 48 h post-treatment. Each bar represents the percentage of dead and alive cells (±SD) assessed in three independent samples. * = p < 0.05; ** = p < 0.01, determined by Student’s t -test.

Journal: Biomolecules

Article Title: Planarian Mucus: A Novel Source of Pleiotropic Cytotoxic and Cytostatic Agents against Cancer Cells

doi: 10.3390/biom14091075

Figure Lengend Snippet: Effect of D. japonica Mucus Treatment on Cell Growth. ( a ) A representative experiment demonstrating the dose-dependent impact of mucus treatment on NCI-H358 and 16HBE14o cell numbers, assessed via crystal violet assay 48 h post-treatment. Each point reflects the mean value (±SD) of three independent samples, normalized to their respective controls. ** = p < 0.01, calculated using Student’s t -test. The inset displays EC50 values determined using the Graphpad Prism program. ( b ) This representative experiment illustrates the influence of various mucus preparations on NCI-H358 cell growth, evaluated through crystal violet assay 48 h post-treatment. Each bar represents the mean absorbance (±SD) of three independent samples. NS indicates not significant, determined by one-way ANOVA. ( c ) A representative experiment illustrating the dose-dependent impact of mucus treatment on cell numbers in A549, Caco-2, and THP-1 cells. Cell numbers were assessed using the crystal violet assay for A549 and Caco-2 cells, and direct counting for THP-1 cells, 48 h post-treatment. Each bar represents the mean value (±SD) of three independent samples, normalized to their respective controls. ** = p < 0.01, calculated by Student’s t -test. ( d ) Representative experiment, in which we observe the dose-dependent effect of mucus treatment on cell death, evaluated using the PI incorporation assay in A549, Caco-2, and THP-1 cells, 48 h post-treatment. Each bar represents the percentage of dead and alive cells (±SD) assessed in three independent samples. * = p < 0.05; ** = p < 0.01, determined by Student’s t -test.

Article Snippet: The human non-small-cell lung cancer cell line A549 and the human bronchioalveolar carcinoma cell line NCI-H358 were kindly provided by Doctor Laura Poliseno from the Italian National Research Council of Pisa.

Techniques: Crystal Violet Assay